Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Electron Microscopy Sciences Pipes Buffer Solution 0.3m, pH 5.5-6.0 500 ML
PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for electron microscopy. It produces excellent results in the preservation of ultrastructural details, especially when long fixation is required. This buffer does not appear to contribute extraneous anions or cations to the tissue. Aqueous PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
pH: 5.5-6.0
Quality Biological Inc Tris HCl (1 M), pH 7.5, PK 4 X 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 500 ML
Please specify pH when ordering.
pH: 6.4-8.0
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 10 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
